Thunder-Link (R) PLUS oligo Antibody Conjugation Kit

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In vitro assay: Thunder-Link PLUS oligo Antibody Labeling Kit [425-0300] - Immuno-PCR data generated using Thunder-Link. A mouse monoclonal antibody specific for human CRP (clone C7) was purchased in unconjugated format ...read more
In vitro assay: Thunder-Link PLUS oligo Antibody Labeling Kit [425-0300] - The graph shows this data following conversion to antigen amount vs. cycle number to enable the calculation of a standard curve. The results ...read more

Product Details

Summary
Applications In vitro

Order Details

Thunder-Link (R) PLUS oligo Antibody Conjugation Kit Summary

Description
Thunder-Link PLUS enables simple and rapid conjugation of antibodies to oligonucleotides, with high recovery of materials and a superior clean-up procedure. The kit is quick and simple to use, overcoming time consuming and lengthy protocols associated with standard conjugation methods.

FeaturesBenefits
Quick and easy to useSave time, no specialist knowledge required
High levels of antibody and oligo recoverySave precious reagents
Use your own oligo and antibody, at your desired ratioFlexible
Freeze driedShips at ambient temperature, long shelf-life
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Unidirectional chemistryNo risk of cross-linking
Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairsCovers the majority of sequences
Linking chemistry works at both 5’ and 3’ endProvides ability to combine with other modifications
Post-conjugation clean-up stepNo interference from unbound oligo
Positive control antibody and oligo includedEnables confirmation of protocol success
Wide range of target proteinsAlso applicable to antibody fragments and small proteins


Advantages of Thunder-Link PLUS:
  • Only 30 minutes antibody and oligo activation
  • Fast oligo conjugation - now only 1 hour!
  • High antibody and oligo recovery
  • Robust and flexible clean-up procedure (now works with antibody fragments and other proteins)
Kit Type
Antibody Conjugation Kit

Applications/Dilutions

Application Notes
Warm the Thunder-Link PLUS Conjugate Clean Up Reagent by placing the tube in warm water (not warmer than 40 deg C) for 10 minutes, then shaking to re-dissolve the contents. Once dissolved, maintain the tube at ~22 deg C to prevent any crystal formation before use.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. 2 or 4 glass vials of Thunder-Link PLUS Oligo Activation Reagent (depending on pack size)
  2. 2 or 4 glass vials of Thunder-Link PLUS Antibody Activation Reagent (depending on pack size)
  3. 1 vial of freeze dried control oligo
  4. 1 vial of freeze dried control antibody
  5. 4 or 8 Separating columns (depending on pack size)
  6. 1 x Thunder-Link PLUS Wash buffer (80 ml or 160 ml depending on pack size)
  7. 1 or 2 vials of Thunder-Link PLUS Conjugate Clean Up Reagent (depending on pack size)
  8. 1 or 2 vials of Thunder-Link PLUS Antibody Suspension buffer (depending on pack size)

Notes

Thunder-Link® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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FAQs for Thunder-Link (R) PLUS oligo Kit (425-0300). (Showing 1 - 1 of 1 FAQ).

  1. We recently ordered a Thunder-Link PLUS oliogo Antibody Conjugation Kit to tag some of our commercial antibodies. The protocol specifies that purified antibodies must be at a concentration of 1mg/mL, as the Activation reagents are designed to activate 100ug of antibody in 100uL reaction volume. Our antibodies are too dilute, at 0.5mg/mL commercial concentration. The protocol suggests contacting technical support. What are your recommendations?
    • We have 2 products designed for antibody concentration, clean up and changing the buffer the antibody is in. These products are Antibody concentration and clean up kit (262-0010) and Antibody concentration and clean up kit (861-0010). But you can use a simple spin column with a 50-100kDa cut off. We do not have these in our portfolio but they are available from various companies e.g Millipore, ThermoFisher, Pierce, Bio-Rad etc. This is very simple and inexpensive way to concentrate your antibody. 

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