Thunder-Link® PLUS oligo Antibody Conjugation Kit Summary
Description |
Thunder-Link PLUS enables simple and rapid conjugation of antibodies to oligonucleotides, with high recovery of materials and a superior clean-up procedure. The kit is quick and simple to use, overcoming time consuming and lengthy protocols associated with standard conjugation methods. Features | Benefits | Quick and easy to use | Save time, no specialist knowledge required | High levels of antibody and oligo recovery | Save precious reagents | Use your own oligo and antibody, at your desired ratio | Flexible | Freeze dried | Ships at ambient temperature, long shelf-life | Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility | Unidirectional chemistry | No risk of cross-linking | Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairs | Covers the majority of sequences | Linking chemistry works at both 5’ and 3’ end | Provides ability to combine with other modifications | Post-conjugation clean-up step | No interference from unbound oligo | Positive control antibody and oligo included | Enables confirmation of protocol success | Wide range of target proteins | Also applicable to antibody fragments and small proteins | Advantages of Thunder-Link PLUS: - Only 30 minutes antibody and oligo activation
- Fast oligo conjugation - now only 1 hour!
- High antibody and oligo recovery
- Robust and flexible clean-up procedure (now works with antibody fragments and other proteins)
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Kit Type |
Antibody Conjugation Kit |
Applications/Dilutions
Dilutions |
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Application Notes |
Warm the Thunder-Link PLUS Conjugate Clean Up Reagent by placing the tube in warm water (not warmer than 40C) for 10 minutes, then shaking to re-dissolve the contents. Once dissolved, maintain the tube at ~22C to prevent any crystal formation before use. |
Reviewed Applications |
Read 1 Review rated 4 using 425-0300 in the following application:
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Packaging, Storage & Formulations
Kit Components
Components
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- 1X Thunder-Link PLUS Wash buffer (80 mL or 160 mL depending on pack size)
- Freeze dried control antibody (1 vial)
- Freeze dried control oligo (1 vial)
- Separating columns (4 or 8 EA depending on pack size)
- Thunder-Link PLUS Antibody Activation Reagent (2 or 4 glass vials depending on pack size)
- Thunder-Link PLUS Antibody Suspension buffer (1 or 2 vials depending on pack size)
- Thunder-Link PLUS Conjugate Clean Up Reagent (1 or 2 vials depending on pack size)
- Thunder-Link PLUS Oligo Activation Reagent (2 or 4 glass vials depending on pack size)
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Notes
Thunder-Link® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Review for Thunder-Link (R) PLUS oligo Kit (425-0300) (1)
41
Average Rating: 4 (Based on 1 review)
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4
reviewed by: Steven Moore
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10/13/2020 |
Summary
Comments
Comments | Il-6 , Il-1b, lps Compared |
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