Thunder-Link® PLUS oligo Antibody Conjugation Kit

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In vitro assay: Thunder-Link (R) PLUS oligo Antibody Conjugation Kit [425-0300] - Immuno-qPCR data generated using Thunder-Link. A mouse monoclonal antibody specific for human CRP (clone C7) was purchased in ...read more
In vitro assay: Thunder-Link (R) PLUS oligo Antibody Conjugation Kit [425-0300] - The graph shows this data following conversion to antigen amount vs. cycle number to enable the calculation of a standard curve. The ...read more

Product Details

Summary
Applications In vitro

Order Details

Thunder-Link® PLUS oligo Antibody Conjugation Kit Summary

Description
Thunder-Link PLUS enables simple and rapid conjugation of antibodies to oligonucleotides, with high recovery of materials and a superior clean-up procedure. The kit is quick and simple to use, overcoming time consuming and lengthy protocols associated with standard conjugation methods.

FeaturesBenefits
Quick and easy to useSave time, no specialist knowledge required
High levels of antibody and oligo recoverySave precious reagents
Use your own oligo and antibody, at your desired ratioFlexible
Freeze driedShips at ambient temperature, long shelf-life
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Unidirectional chemistryNo risk of cross-linking
Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairsCovers the majority of sequences
Linking chemistry works at both 5’ and 3’ endProvides ability to combine with other modifications
Post-conjugation clean-up stepNo interference from unbound oligo
Positive control antibody and oligo includedEnables confirmation of protocol success
Wide range of target proteinsAlso applicable to antibody fragments and small proteins


Advantages of Thunder-Link PLUS:
  • Only 30 minutes antibody and oligo activation
  • Fast oligo conjugation - now only 1 hour!
  • High antibody and oligo recovery
  • Robust and flexible clean-up procedure (now works with antibody fragments and other proteins)
Kit Type
Antibody Conjugation Kit

Applications/Dilutions

Application Notes
Warm the Thunder-Link PLUS Conjugate Clean Up Reagent by placing the tube in warm water (not warmer than 40C) for 10 minutes, then shaking to re-dissolve the contents. Once dissolved, maintain the tube at ~22C to prevent any crystal formation before use.
Reviewed Applications
Read 2 Reviews rated 4
using
425-0300 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Separating columns (4 or 8 EA depending on pack size)
  2. Thunder-Link PLUS Antibody Suspension buffer (1 or 2 vials depending on pack size)
  3. Thunder-Link PLUS Conjugate Clean Up Reagent (1 or 2 vials depending on pack size)
  4. Thunder-Link PLUS Oligo Activation Reagent (2 or 4 glass vials depending on pack size)
  5. Thunder-Link PLUS Antibody Activation Reagent (2 or 4 glass vials depending on pack size)
  6. Freeze dried control oligo (1 vial)
  7. Freeze dried control antibody (1 vial)
  8. 1X Thunder-Link PLUS Wash buffer (80 mL or 160 mL depending on pack size)

Notes

Thunder-Link® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Reviews for Thunder-Link (R) PLUS oligo Kit (425-0300) (2) 42

Average Rating: 4
(Based on 2 reviews)

Reviews using 425-0300:
Filter by Applications
ICC/IF
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence Thunder-Link (R) PLUS oligo 425-0300
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4
reviewed by:
Steven Moore
ICC/IF 10/13/2020
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lot425-0300

Comments

CommentsIl-6, Il-1b, lps Compared
Serum Thunder-Link (R) PLUS oligo 425-0300
Enlarge
4
reviewed by:
Steven Moore
10/13/2020
View

Summary

ApplicationSerum
Lot425-0300

Comments

CommentsIl-6, Il-1b, lps Compared

FAQs for Thunder-Link (R) PLUS oligo Kit (425-0300). (Showing 1 - 2 of 2 FAQ).

  1. If I want to conjugate an oligo to my antibody using your kit (Thunder-link Plus kit), what type of modification needs to be in my oligo.
    • The oligo must be between 10 and 120 base pairs in length and contain a terminal amine group, which must be added during synthesis. All commercial oligo suppliers offer this modification. The efficiency of conjugation is slightly higher with 5’aminated oligos.
  2. We recently ordered a Thunder-Link PLUS oliogo Antibody Conjugation Kit to tag some of our commercial antibodies. The protocol specifies that purified antibodies must be at a concentration of 1mg/mL, as the Activation reagents are designed to activate 100ug of antibody in 100uL reaction volume. Our antibodies are too dilute, at 0.5mg/mL commercial concentration. The protocol suggests contacting technical support. What are your recommendations?
    • We have 2 products designed for antibody concentration, clean up and changing the buffer the antibody is in. These products are Antibody concentration and clean up kit (262-0010) and Antibody concentration and clean up kit (861-0010). But you can use a simple spin column with a 50-100kDa cut off. We do not have these in our portfolio but they are available from various companies e.g Millipore, ThermoFisher, Pierce, Bio-Rad etc. This is very simple and inexpensive way to concentrate your antibody. 

Other Available Formats

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