Thomsen-Friedenreich Antigen Antibody (A84-A/F10)

Immunohistochemistry-Paraffin: Thomsen-Friedenreich Antigen Antibody (A84-A/F10) [NBP3-07630] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Thomsen-Friedenreich Antigen Mouse Monoclonal Antibody (A84-A/F10).

Immunohistochemistry-Paraffin: Thomsen-Friedenreich Antigen Antibody (A84-A/F10) [NBP3-07630] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Thomsen-Friedenreich Antigen Mouse Monoclonal Antibody (A84-A/F10).

Product Discontinued

Thomsen-Friedenreich Antigen Antibody (A84-A/F10) Summary

• Immunogen: Neuraminidase-treated human red blood cells
• Localization: Cell Surface
• Specificity: This antibody recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid), and shows no cross-reactivity with sialylated glycophorin.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein L or PEG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at 37C. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein L or PEG purified

Alternate Names for Thomsen-Friedenreich Antigen Antibody (A84-A/F10)

• TFA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.