Thioguanine (2-Amino-6-Mercaptopurine) Antibody (5B2)

Product Discontinued

Thioguanine (2-Amino-6-Mercaptopurine) Antibody (5B2) Summary

• Immunogen: 9-substituted thioguanine linked to PPD (2)
• Specificity: This has specificity for thioguanine. Cross reactivity is seen with a variety of thioguanine analogues, except with 6-(methylthio)purine, 6-(methylthio)purine riboside, 6-thioxanthine and 2-amino-8-phenyl-6-purinethione and (2).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Background

Thioguanine is a purine analogue used in cancer chemotherapy. It is the 6-thione analogue of the endogenous 6-OHpurine base guanine. In the cell thioguanine is converted to the ribonucleotides: 6-thioguanosine-5'-phosphate and 6-thioinosine-5'-phosphate. These fraudulent nucleotides produce their cytotoxic effects by several mechanisms, e.g. inhibitory action on de novo purine synthesis, and may themselves be incorporated into DNA. Thioguanine has a molecular weight of 167 Da (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.