Th1/Th2 Cytokines Bead-Based 5-Plex Flow Cytometry Kit

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP3-45144
    • Availability
      Product Discontinued

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Th1/Th2 Cytokines Bead-Based 5-Plex Flow Cytometry Kit Summary

Description

This kit utilizes antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

Assay Length: 3 h

Excitation/Emission (nm): 575/495,565

UniProt
IL-2: P04351
IFN-gamma: P01580
IL-4: P07750
IL-5: P04401
TNF-alpha: P06804

Specificity
This kit is for detection of IFN-gamma, IL-2, IL-4, IL-5 and TNF-alpha in mouse serum, plasma, biological fluid and cell culture supernatant.
Sensitivity
IL-2 < 2pg/mL
IFN-gamma < 0.5pg/mL
IL-4 < 0.5pg/mL
IL-5 < 5pg/mL
TNF-alpha < 0.5pg/mL
Assay Type
Multi-Plex detection combination
Inter-Assay
%CV < 20
Intra-Assay
%CV < 10
Spike Recovery
70 - 130%
Sample Volume
15 uL
Kit Type
Flow Cytometry Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry

Reactivity Notes

Cross-reactivity of analytes in the Panel: Negligible

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 x Premixed Antibody-Conjugated Beads
  2. 1 x SA-PE
  3. 10 x Reading Buffer
  4. 10 x Wash Buffer
  5. 2 x Detection Antibody Diluent
  6. 2 x Premixed Biotinylated Detection Antibodies
  7. Lyophilized Antigen Standard
  8. Manual
  9. Plate Sealer
  10. SPB Assay Buffer
  11. SPB Standard Diluent

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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