Th1/Th2 Cytokines Bead-Based 5-Plex Flow Cytometry Kit

Product Discontinued

Th1/Th2 Cytokines Bead-Based 5-Plex Flow Cytometry Kit Summary

• Description:

  This kit utilizes antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

  Assay Length: 3 h

  Excitation/Emission (nm): 575/495,565

  UniProt
  IL-2: P04351
  IFN-gamma: P01580
  IL-4: P07750
  IL-5: P04401
  TNF-alpha: P06804

• Specificity: This kit is for detection of IFN-gamma, IL-2, IL-4, IL-5 and TNF-alpha in mouse serum, plasma, biological fluid and cell culture supernatant.
• Sensitivity: IL-2 < 2pg/mL IFN-gamma < 0.5pg/mL IL-4 < 0.5pg/mL IL-5 < 5pg/mL TNF-alpha < 0.5pg/mL
• Assay Type: Multi-Plex detection combination
• Inter-Assay: %CV < 20
• Intra-Assay: %CV < 10
• Spike Recovery: 70 - 130%
• Sample Volume: 15 uL
• Kit Type: Flow Cytometry Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Reactivity Notes

Cross-reactivity of analytes in the Panel: Negligible

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.