TGF-beta 1/1.2 Antibody [Unconjugated]

Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF‑ beta 1/1.2 Polyclonal Antibody (Catalog # AB-101-NA). The ND50 is typically 0.2-0.6 µg/mL.

• Reactivity: All-Multi
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: LYOPH

TGF-beta 1/1.2 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B)
  Ala279-Ser390
  Accession # P01137
• Specificity: Detects TGF‑ beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant amphibian TGF‑ beta 5 is observed and less than 2% cross-reactivity with recombinant human TGF‑ beta 3, recombinant chicken TGF‑ beta 3, and porcine TGF‑ beta 2 is observed.
• Source: N/A
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Purity Statement: Ion exchange-purified from egg yolks
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-0.6 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 1/1.2 Antibody [Unconjugated]

• TGFb1.2
• TGF-beta 1/1.2

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1/1.2 Antibody (AB-101-NA)(12)

Publications using AB-101-NA

• W Singhatana, S Kitpakorns, M Toso, P Pavasant IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact Royal Society open science, 2022-07-27;9(7):220056. 2022-07-27 [PMID: 35911203] (Neutralization, Human)
• M Popek, B Bobula, K Orze?-Gajo, M Zieli?ska The Effect of TGF-beta1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy International Journal of Molecular Sciences, 2022-01-19;23(3):. 2022-01-19 [PMID: 35163004] (Neutralization, Mouse)
• Cody C. Gifford, Fei Lian, Jiaqi Tang, Angelica Costello, Roel Goldschmeding, Rohan Samarakoon et al. PAI‐1 induction during kidney injury promotes fibrotic epithelial dysfunction via deregulation of klotho, p53, and TGF‐ beta 1‐receptor signaling The FASEB Journal 2021-11-08 [PMID: 34110636]
• A Sacchi, G Grassi, V Bordoni, P Lorenzini, E Cimini, R Casetti, E Tartaglia, L Marchioni, N Petrosillo, F Palmieri, G D'Offizi, S Notari, M Tempestill, MR Capobianch, E Nicastri, M Maeurer, A Zumla, F Locatelli, A Antinori, G Ippolito, C Agrati Early expansion of myeloid-derived suppressor cells inhibits SARS-CoV-2 specific T-cell response and may predict fatal COVID-19 outcome Cell Death Dis, 2020-10-27;11(10):921. 2020-10-27 [PMID: 33110074] (Neutralization, Human)
• Amanda C Brodeur, Anna M Roberts-Pilgrim, Kimberlee L Thompson, Craig L Franklin, Charlotte L Phillips Transforming growth factor-&beta;1/Smad3-independent epithelial&ndash;mesenchymal transition in type I collagen glomerulopathy International Journal of Nephrology and Renovascular Disease 2017-09-19 [PMID: 28919801]
• S S Prime Cancer associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-�-dependent pathways in genotype-specific oral cancer Carcinogenesis, 2016-11-01;0(0):. 2016-11-01 [PMID: 27803052] (Neutralization, Human)
• van den Berk L, Jansen B, Snowden S, Siebers-Vermeulen K, Gilissen C, Kogler G, Figdor C, Wheelock C, Torensma R Cord blood mesenchymal stem cells suppress DC-T Cell proliferation via prostaglandin B2. Stem Cells Dev, 2014-04-23;23(14):1582-93. 2014-04-23 [PMID: 24649980] (Neutralization, Human)
• Sheena D. Brown, Lou Ann S. Brown Ethanol (EtOH)-induced TGF-beta 1 and reactive oxygen species production are necessary for EtOH-induced alveolar macrophage dysfunction and induction of alternative activation Alcoholism: Clinical and Experimental Research 2012-11-01 [PMID: 22551312]
• Rosalyn A. Jurjus, Yueyuan Liu, Sonali Pal-Ghosh, Gauri Tadvalkar, Mary Ann Stepp ORIGINAL RESEARCH - BASIC SCIENCE: Primary dermal fibroblasts derived from sdc-1 deficient mice migrate faster and have altered alpha v integrin function Wound Repair and Regeneration 2013-05-15 [PMID: 19128260]
• Ciriaco A. Piccirillo, John J. Letterio, Angela M. Thornton, Rebecca S. McHugh, Mizuko Mamura, Hidekazu Mizuhara et al. CD4(+)CD25(+) regulatory T cells can mediate suppressor function in the absence of transforming growth factor beta1 production and responsiveness The Journal of Experimental Medicine 2002-07-15 [PMID: 12119348] (Neutralization, Mouse)
• WanJun Chen, Wenwen Jin, Hongsheng Tian, Paula Sicurello, Mark Frank, Jan M. Orenstein et al. Requirement for Transforming Growth Factor beta 1 in Controlling T Cell Apoptosis J. Exp. Med 2001-08-20 [PMID: 11514601]
• Mozes MM, Hodics T, Kopp JB Isoform specificity of commercially-available anti-TGF-beta antibodies. J. Immunol. Methods, 1999-05-27;225(1):87-93. 1999-05-27 [PMID: 10365785] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • P01137(Human)