Tetrahydrobiopterin ELISA Kit (Colorimetric)

ELISA: Tetrahydrobiopterin ELISA Kit (Colorimetric) [NBP2-74983] - Samples were spiked with high concentrations of Tetrahydrobiopterin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Tetrahydrobiopterin ELISA Kit (Colorimetric) [NBP2-74983] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Tetrahydrobiopterin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Tetrahydrobiopterin in samples. No significant cross-reactivity or interference between Tetrahydrobiopterin and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 5.43%
• Intra-Assay: CV% < 5.7%
• Spike Recovery: 88-108%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Tetrahydrobiopterin ELISA Kit (NBP2-74983)(2)

Publications using NBP2-74983

• Mohd Sabri NA, Lee SK, Murugan DD, Ling WC Epigallocatechin gallate (EGCG) alleviates vascular dysfunction in angiotensin II-infused hypertensive mice by modulating oxidative stress and eNOS Scientific reports 2022-10-21 [PMID: 36271015] (ELISA)
• Garate-Carrillo A, Navarrete-Yanez V, Oritiz-Vilchis P et al. Arginase inhibition by (-)-Epicatechin reverses endothelial cell aging Eur J Pharmacol 2020-08-11 [PMID: 32795514] (ELISA, Bovine)

FAQs for Tetrahydrobiopterin ELISA Kit (NBP2-74983). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.