Rat Testosterone ELISA Kit (Colorimetric)

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Rat Testosterone ELISA Kit (Colorimetric) [NBP3-23568] - Samples were spiked with high concentrations of Rat Testosterone and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat Testosterone ELISA Kit (Colorimetric) [NBP3-23568] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.07 ng/mL

Order Details

Rat Testosterone ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Testosterone in samples. No significant cross-reactivity or interference between Rat Testosterone and analogues was observed.
Standard Curve Range
0.16-10 ng/mL
Sensitivity
0.07 ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
%CV < 5.48
Intra-Assay
%CV < 5.82
Spike Recovery
86-102%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Concentrated HRP Linked Detection Ab (100x)
  2. Concentrated Wash Buffer (25x)
  3. HRP Linked Ab Diluent
  4. Micro ELISA Plate (Dismountable)
  5. Plate Sealer
  6. Product Manual
  7. Reference Standard
  8. Sample Diluent
  9. Stop Solution
  10. Substrate Reagent

Alternate Names for Rat Testosterone ELISA Kit (Colorimetric)

  • 17b-Hydroxyandrost-4-ene-3-one
  • 17b-Testosterone
  • Testosterone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Testosterone ELISA Kit (NBP3-23568). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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