Testosterone ELISA Kit (Colorimetric)

ELISA: Testosterone ELISA Kit (Colorimetric) [NBP2-42044] - Standard curve using NBP2-42044 Testosterone ELISA Kit

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Testosterone ELISA Kit (Colorimetric) Summary

• Specificity: Crossreactivity summary to various steroid hormones:
  Testosterone (100%), 19-hydroxytestosterone (14.6%), Androstendione (7.20%), Dehydroepiandrosterone (0.72%), Estradiol (0.40%) and <0.001%: Dihydrotestosterone, Estriol, Aldosterone, Corticosterone, Cortisol, Cortisone, Estrone, Progesterone, Pregenolone
• Standard Curve Range: 7.81 - 2,000 pg/ml
• Sensitivity: 5.67 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV = 14.6%
  Medium Concentration %CV = 11.3%
  High Concentration %CV = 9.3%
• Intra-Assay: Low Concentration %CV = 10.8%
  Medium Concentration %CV = 10%
  High Concentration %CV = 7.8%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: For the quantitative determination of free or total testosterone in culture supernatants, plasma, serum, and saliva from any species. Not suitable for human serum and plasma.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Testosterone ELISA Kit (Colorimetric)

• 17b-Hydroxyandrost-4-ene-3-one
• 17b-Testosterone
• Testosterone

Background

Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Testosterone ELISA Kit (NBP2-42044)(1)

Publication using NBP2-42044

• Zhu J, Yang Q, Li H et al. Sirt3 deficiency accelerates ovarian senescence without affecting spermatogenesis in aging mice Free radical biology & medicine 2022-11-03 [PMID: 36336229]

FAQs for Testosterone ELISA Kit (NBP2-42044). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.