Testosterone ELISA Kit (Colorimetric)

ELISA: Testosterone ELISA Kit (Colorimetric) [NBP2-42044] - Standard curve using NBP2-42044 Testosterone ELISA Kit

Product Discontinued

Testosterone ELISA Kit (Colorimetric) Summary

• Specificity: Crossreactivity summary to various steroid hormones:
  Testosterone (100%), 19-hydroxytestosterone (14.6%), Androstendione (7.20%), Dehydroepiandrosterone (0.72%), Estradiol (0.40%) and <0.001%: Dihydrotestosterone, Estriol, Aldosterone, Corticosterone, Cortisol, Cortisone, Estrone, Progesterone, Pregenolone
• Standard Curve Range: 7.81 - 2,000 pg/ml
• Sensitivity: 5.67 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV = 14.6%
  Medium Concentration %CV = 11.3%
  High Concentration %CV = 9.3%
• Intra-Assay: Low Concentration %CV = 10.8%
  Medium Concentration %CV = 10%
  High Concentration %CV = 7.8%
• Spike Recovery: Please See Protocol for Specific Spike Recovery Details
• Sample Volume: 0.1 ml
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: For the quantitative determination of free or total testosterone in culture supernatants, plasma, serum, and saliva from any species. Not suitable for human serum and plasma.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Testosterone ELISA Kit (Colorimetric)

• 17b-Hydroxyandrost-4-ene-3-one
• 17b-Testosterone
• Testosterone

Background

Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Testosterone ELISA Kit (NBP2-42044)(1)

Publication using NBP2-42044

• Zhu J, Yang Q, Li H et al. Sirt3 deficiency accelerates ovarian senescence without affecting spermatogenesis in aging mice Free radical biology & medicine 2022-11-03 [PMID: 36336229]

FAQs for Testosterone ELISA Kit (NBP2-42044). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.