Human Testis Whole Tissue Lysate (Adult Whole Tumor)

• Reactivity: Hu
• Applications: WB
• Concentration: 5.0 mg/ml

Human Testis Whole Tissue Lysate (Adult Whole Tumor) Summary

• Description: We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
• Specificity: Total Protein - Human Tumor Tissue: Testis

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Reproductive
• Protein State: Native
• Tissue Condition: Tumor
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Testis Lysate (NB820-59455). (Showing 1 - 2 of 2 FAQ).

1. I wondered if you could provide some more information regarding sample NB820-59455. Is this tissue extract from the whole testis of a testis tumor patient or just from a testis tumor specifically?
   • This lysate was prepared from a 30 year old male testis tumor. The type of tumor or any further information is not known.
2. I have some questions regarding the whole humor testis lysate with the product number: NB820-59455. Which type of tumor was is it specifically and do you have information about the extent of it? And is the cell lysate just from the testis tumor or the whole testis including non-tumor tissue?
   • The lab have informed me that the current lot is Seminoma. None of our tumor lysate can be claimed to have 100% tumor cells, there are always normal cells included in the cancer tissues. Average tumor cell should be >70%. Unfortunately we do not have any further information.