Tangential Flow Filtration (TFF)-Small EV Isolation

• Suitable Sample Type: EV containing matrices (urine, cell culture supernatant, plasma, serum)

Tangential Flow Filtration (TFF)-Small EV Isolation Summary

• Description:

  Tangential flow filter-EV are a filter cartridge of hollow polyethersulfone fibers used for purification and concentration of extracellular vesicles. The filter allows a rapid, reproducible and scalable purification of EVs. It can be used on the lab bench for purifying small volume of samples or connected with a mechanical system for purifying larger volumes. During the purification, the sample is simultaneously concentrated.

• Sample Volume: 10 mL - 1000 mL

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Background

Tangetial flow filtration (TFF) is a technique primarily used for concentrating large sample volumes containing extracellular vesicles (EVs). The process involves passing the EV-containing liquid horizontally (tangentially) over the pores of the membrane allowing particles that are smaller than the membrane pores to filter through, while larger particles are retained in the chamber.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.