Tangential Flow Filtration (TFF)- MV Filter

• Suitable Sample Type: Diluted matrices (urine and cell culture supernatant)

Tangential Flow Filtration (TFF)- MV Filter Summary

• Description:

  Tangential flow filter-MV are a filter cartridge of hollow polysulfone fibers used for separating different EVs by size. Microvesicles bigger than 150 nm are retained inside the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered with a syringe in PBS buffer without additional purification steps.

• Sample Volume: 10 mL up to several liters if connected to a mechanical pump

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Background

Tangetial flow filtration (TFF) is a technique primarily used for concentrating large sample volumes containing extracellular vesicles (EVs). The process involves passing the EV-containing liquid horizontally (tangentially) over the pores of the membrane allowing particles that are smaller than the membrane pores to filter through, while larger particles are retained in the chamber.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.