T7 Epitope Tag Antibody

Western Blot: T7 Epitope Tag Antibody [NB600-372] - Detection of T7-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody NB600-372 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.

Product Discontinued

T7 Epitope Tag Antibody Summary

• Immunogen: Rabbits were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Rabbit anti- human T7 affinity purified antibodies were coupled to peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Primary-1:1000-1:30000; Coating: 1:100-1:500
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1 - 4 ug/mg lysate
  • Western Blot 1:1000-1:30000

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 29689197).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for T7 Epitope Tag Antibody

• MASMTGGQQMG epitope tag
• MASMTGGQQMG tag
• T7 epitope tag

Background

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T7 Epitope Tag Antibody (NB600-372)(15)

Publications using NB600-372

• Carbonell, C;Ulsamer, A;Vivori, C;Papasaikas, P;Bottcher, R;Joaquin, M;Minana, B;Tejedor, JR;de Nadal, E;Valcarcel, J;Posas, F; Functional Network Analysis Reveals the Relevance of SKIIP in the Regulation of Alternative Splicing by p38 SAPK Cell Rep 2019-04-16 [PMID: 30995481] (WB)
• Tan Kai Li, Haelterman Nele A, Kwartler Callie S et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. PloS One 2019-04-23 [PMID: 29689197] (WB, Drosophila)
• Saribas AS, White MK, Safak M. Structure-based release analysis of the JC virus agnoprotein regions: A role for the hydrophilic surface of the major alpha helix domain in release. J Cell Physiol. 2017-07-19 [PMID: 28722139] (IP)
  
  Details:
  This citation used the Agarose Immobilized form of this antibody.
• Tejedor JR, Tilgner H, Iannone C et al. Role of six single nucleotide polymorphisms, risk factors in coronary disease, in OLR1 alternative splicing. RNA 2015-06-01 [PMID: 25904137] (WB)
• Zhou J, Liu Y, Zhang W et al. Transcription elongation regulator 1 is a co-integrator of the cell fate determination factor Dachshund homolog 1. J Biol Chem 2010-12-01 [PMID: 20956529]
• Rippa V, Cirulli C, Di Palo B et al. The ribosomal protein L2 interacts with the RNA polymerase alpha subunit and acts as a transcription modulator in Escherichia coli J Bacteriol 2010-04-01 [PMID: 20097853]
  
  Details:
  This citation used the Agarose Immobilized version of this antibody.
• Sevova ES, Bangs JD. Streamlined architecture and glycosylphosphatidylinositol-dependent trafficking in the early secretory pathway of African trypanosomes. Mol Biol Cell 2009-11-01 [PMID: 19759175]
• Rayala SK, Martin E, Sharina IG et al. Dynamic interplay between nitration and phosphorylation of tubulin cofactor B in the control of microtubule dynamics. Proc Natl Acad Sci U S A 2007-12-01 [PMID: 18048340]
• Gururaj AE, Peng S, Vadlamudi RK et al. Estrogen induces expression of BCAS3, a novel estrogen receptor-alpha coactivator, through proline-, glutamic acid-, and leucine-rich protein-1 (PELP1). Mol Endocrinol 2007-08-01 [PMID: 17505058]
• Manavathi B, Rayala SK, Kumar R et al. Phosphorylation-dependent regulation of stability and transforming potential of ETS transcriptional factor ESE-1 by p21-activated kinase 1. J Biol Chem 2007-07-01 [PMID: 17491012]
• Miura K, Jin JB, Lee J et al. SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis. Plant Cell 2007-04-01 [PMID: 17416732]
• Lee PC, Nguyen AD, Debose-Boyd RA et al. Mutations within the membrane domain of HMG-CoA reductase confer resistance to sterol-accelerated degradation. J Lipid Res 2007-02-01 [PMID: 17090658]
• Singh RR, Gururaj AE, Vadlamudi RK et al. 9-cis-retinoic acid up-regulates expression of transcriptional coregulator PELP1, a novel coactivator of the retinoid X receptor alpha pathway. J Biol Chem 2006-06-01 [PMID: 16574651]
• Singh RR, Barnes CJ, Talukder AH et al. Negative regulation of estrogen receptor alpha transactivation functions by LIM domain only 4 protein. Cancer Res 2005-11-01 [PMID: 16288053]
• Singh A, Karnoub AE, Palmby TR et al. Rac1b, a tumor associated, constitutively active Rac1 splice variant, promotes cellular transformation. Oncogene 2004-12-01 [PMID: 15516977]