SW480 Whole Cell Lysate

Images

 
There are currently no images for SW480 Lysate (NBP2-03966).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2 mg/ml

Order Details

SW480 Whole Cell Lysate Summary

Specificity
SW480 (Human Colorectal Adenocarcinoma)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Supplied in SDS sample buffer containing 5% Beta-mercaptoethanol.
Concentration
2 mg/ml

Lysate Details for SW480

Type
Cell
Tissue
Digestive/Waste
Subcellular Fraction
Whole

Notes

GROWTH MEDIA: ATCC medium (Leibovitz's L-15 medium with 2 mM L-glutamine, and 10% FBS)

Background

SW480 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The SW480 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for SW480 Lysate (NBP2-03966) (0)

There are no publications for SW480 Lysate (NBP2-03966).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SW480 Lysate (NBP2-03966) (0)

There are no reviews for SW480 Lysate (NBP2-03966). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SW480 Lysate (NBP2-03966) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SW480 Products

SW480 NBP2-03966

Blogs on SW480

There are no specific blogs for SW480, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SW480 Whole Cell Lysate and receive a gift card or discount.