STRO-1 Antibody (STRO-1) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
STRO-1
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

STRO-1 Antibody (STRO-1) [FITC] Summary

Immunogen
Human CD34+ bone marrow cells.
Marker
Stromal Cell Surface Marker
Isotype
IgM Lambda
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This STRO-1 Antibody is useful for Immunocytochemistry/Immunofluorescence and Flow Cytometry.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
IgM purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for STRO-1 Antibody (STRO-1) [FITC]

  • STRO1
  • STRO-1

Background

As a part of experimental investigations focused on identifying reliable markers for mesenchymal stromal/stem cells (MSC), STRO-1 antibody was generated by using one of the several hybridomas that were generated by immunizing mice intrasplenically with human CD34+ bone marrow cells and despite lack of much details on exact identity of its cognate antigen, STRO-1 antibody has been heavily relied upon for the recognition/isolation of various types of MSCs for over past two decades. After initial screening against T-/B-cell lines, hybridomas followed further selection for reactivity against subpopulations of CD34-expressing cells as well as for the identification of STRO-1 clone, and additional tests identified STRO-1 antibody as of the IgM isotype which reacts with stromal cells in the adherent layer of long-term bone marrow cultures. STRO-1 has been considered the best-known MSC marker and its antibody has been employed primarily for FLOW application as well as for staining of putative MSCs. However, with the use of IHC and Western blot applications, recent studies have identified STRO-1 as a 75kd endothelial antigen in the vasculature of liver, lung and kidney tissues wherein it co-localized with endothelial marker vWF and also detected it in certain cancer cell lines namely PC3, DU145, MCF7, and K562.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for STRO-1 Antibody (NBP1-48356F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-48356APC
Biotin Labeled NBP1-48356B
DyLight 350 Labeled NBP1-48356UV
DyLight 405 Labeled NBP1-48356V
DyLight 488 Labeled NBP1-48356G
DyLight 550 Labeled NBP1-48356R
DyLight 650 Labeled NBP1-48356C
DyLight 680 Labeled NBP1-48356FR
DyLight 755 Labeled NBP1-48356IR
PE Labeled NBP1-48356PE
PerCP Labeled NBP1-48356PCP

Additional STRO-1 Products

STRO-1 NBP1-48356F

Research Areas for STRO-1 Antibody (NBP1-48356F)

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