Streptolysin Antibody (6D11) - BSA Free

Images

 
Western Blot: Streptolysin Antibody (6D11) - BSA Free [NBP1-05126] - Activation receptors are shed during intrinsic repair. BMDM were unchallenged (control) or challenged with 500 HU/mL SLO WT, PFO or an equivalent mass ...read more

Product Details

Summary
Reactivity Hu, All-NA, BaSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Streptolysin Antibody (6D11) - BSA Free Summary

Immunogen
Cysteine chloride oxidized streptolysin
Specificity
Specific for oxidized streptolysin but cross react to untreated streptolysin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:400
  • Immunocytochemistry/Immunofluorescence
Application Notes
Use in Western blot and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28186501).
Reviewed Applications
Read 3 Reviews rated 4
using
NBP1-05126 in the following applications:

Publications
Read Publications using
NBP1-05126 in the following applications:

Reactivity Notes

Chemical. Human reactivity reported in scientific literature (PMID: 28186501).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Streptolysin Antibody (6D11) - BSA Free

  • Slo
  • Thiol activated cytolysin

Background

Bacteria of the Streptococcus genera produce the exotoxin streptolysin. Anti-streptolysin antibody titers in patients are measured as a marker of inflammation. Streptolysin is also an important tool in cell biology due to its hemolytic activity and the ability to permeabilize plasma membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Streptolysin Antibody (NBP1-05126)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Streptolysin Antibody (NBP1-05126) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Other.

Reviews using NBP1-05126:
Filter by Applications
IF
(1)
WB
(2)
All Applications
Filter by Species
Other
(3)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Streptolysin NBP1-05126
Enlarge
3
reviewed by:
Verified Customer
IF Other 04/02/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells challenged with Streptolysin O
SpeciesOther
LotG0620

Blocking

Blocking Details10% goat serum with 0.1% Triton X-100 in PBS for 30 min room temp

Primary Anitbody

Dilution Ratio1:250 in 10% goat serum, 0.1% Triton X-100 in PBS for 1 hr room temp.

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG AlexaFluor488
Secondary Manufacturer Cat#Thermofisher A-11029
Secondary Concentration1:500 (4 ug/mL)

Details

Detection NotesImaged with 60x 1.40 NA objective on Olympus Fluoview 300 confocal microscope using 488 nm and 543 nm lines
Fixation Details2% para-formaldehyde in PBS for 15 min room temp
Wash Description3x PBS

Comments

CommentsSLO in greenDextran in red
Western Blot Streptolysin NBP1-05126
Enlarge
4
reviewed by:
Verified Customer
WB Other 04/02/2016
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant Perfringolysin O
SpeciesOther
LotG0620

Blocking

Blocking Details1 hr room temp in 5% milk in Tris-buffered saline with 0.1% Tween20

Primary Anitbody

Dilution Ratio1:2000 for 2 h at room temp in 1% milk in Tris-buffered saline with 0.1% Tween20

Secondary Antibody

Secondary DescriptionDonkey anti-Mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch 715-035-151
Secondary Concentration1:10,000

Details

Detection NotesBands were detected on a FluorChemE with 2 min exposure.
Western Blot Streptolysin NBP1-05126
Enlarge
5
reviewed by:
Verified Customer
WB Other 04/01/2016
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant Streptolysin O
SpeciesOther
LotG0620

Blocking

Blocking Details1 hr room temp in 5% milk in Tris-buffered saline with 0.1% Tween20

Primary Anitbody

Dilution Ratio1:2000 for 2 h at room temp in 1% milk in Tris-buffered saline with 0.1% Tween20

Secondary Antibody

Secondary DescriptionDonkey anti-Mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch 715-035-151
Secondary Concentration1:10,000

Details

Detection NotesBands were detected on a FluorChemE with 2 min exposure.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Streptolysin Antibody (NBP1-05126) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

Verified Customer
04/02/2016
Application: IF
Species: Other

Verified Customer
04/02/2016
Application: WB
Species: Other

Verified Customer
04/01/2016
Application: WB
Species: Other