Streptolysin Antibody (6D11) - BSA Free

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Product Details

Summary
Reactivity Hu, Ba, NASpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Streptolysin Antibody (6D11) - BSA Free Summary

Immunogen
Cysteine chloride oxidized streptolysin
Specificity
Specific for oxidized streptolysin but cross react to untreated streptolysin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:400
  • Immunocytochemistry/Immunofluorescence
Application Notes
Use in Western blot and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28186501).
Reviewed Applications
Read 3 Reviews rated 4
using
NBP1-05126 in the following applications:

Publications
Read Publications using
NBP1-05126 in the following applications:

Reactivity Notes

Chemical. Human reactivity reported in scientific literature (PMID: 28186501).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Streptolysin Antibody (6D11) - BSA Free

  • Slo
  • Thiol activated cytolysin

Background

Bacteria of the Streptococcus genera produce the exotoxin streptolysin. Anti-streptolysin antibody titers in patients are measured as a marker of inflammation. Streptolysin is also an important tool in cell biology due to its hemolytic activity and the ability to permeabilize plasma membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Streptolysin Antibody (NBP1-05126)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Streptolysin Antibody (NBP1-05126) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Other.

Reviews using NBP1-05126:
Filter by Applications
IF
(1)
WB
(2)
All Applications
Filter by Species
Other
(3)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Streptolysin NBP1-05126
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reviewed by:
Anonymous
IF Other 04/02/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells challenged with Streptolysin O
SpeciesOther
LotG0620

Blocking

Blocking Details10% goat serum with 0.1% Triton X-100 in PBS for 30 min room temp

Primary Anitbody

Dilution Ratio1:250 in 10% goat serum, 0.1% Triton X-100 in PBS for 1 hr room temp.

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG AlexaFluor488
Secondary Manufacturer Cat#Thermofisher A-11029
Secondary Concentration1:500 (4 ug/mL)

Details

Detection NotesImaged with 60x 1.40 NA objective on Olympus Fluoview 300 confocal microscope using 488 nm and 543 nm lines
Fixation Details2% para-formaldehyde in PBS for 15 min room temp
Wash Description3x PBS

Comments

CommentsSLO in greenDextran in red
Western Blot Streptolysin NBP1-05126
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reviewed by:
Anonymous
WB Other 04/02/2016
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant Perfringolysin O
SpeciesOther
LotG0620

Blocking

Blocking Details1 hr room temp in 5% milk in Tris-buffered saline with 0.1% Tween20

Primary Anitbody

Dilution Ratio1:2000 for 2 h at room temp in 1% milk in Tris-buffered saline with 0.1% Tween20

Secondary Antibody

Secondary DescriptionDonkey anti-Mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch 715-035-151
Secondary Concentration1:10,000

Details

Detection NotesBands were detected on a FluorChemE with 2 min exposure.
Western Blot Streptolysin NBP1-05126
Enlarge
reviewed by:
Anonymous
WB Other 04/01/2016
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant Streptolysin O
SpeciesOther
LotG0620

Blocking

Blocking Details1 hr room temp in 5% milk in Tris-buffered saline with 0.1% Tween20

Primary Anitbody

Dilution Ratio1:2000 for 2 h at room temp in 1% milk in Tris-buffered saline with 0.1% Tween20

Secondary Antibody

Secondary DescriptionDonkey anti-Mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch 715-035-151
Secondary Concentration1:10,000

Details

Detection NotesBands were detected on a FluorChemE with 2 min exposure.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Streptolysin Antibody (NBP1-05126) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-05126AF350
Alexa Fluor 405 NBP1-05126AF405
Alexa Fluor 488 NBP1-05126AF488
Alexa Fluor 532 NBP1-05126AF532
Alexa Fluor 594 NBP1-05126AF594
Alexa Fluor 647 NBP1-05126AF647
Alexa Fluor 700 NBP1-05126AF700
Alexa Fluor 750 NBP1-05126AF750
Biotin NBP1-05126B
DyLight 350 NBP1-05126UV
DyLight 405 NBP1-05126V
DyLight 488 NBP1-05126G
DyLight 550 NBP1-05126R
DyLight 594 NBP1-05126DL594
DyLight 650 NBP1-05126C
DyLight 680 NBP1-05126FR
DyLight 755 NBP1-05126IR
FITC NBP1-05126F
HRP NBP1-05126H

Additional Streptolysin Products

Blogs on Streptolysin

There are no specific blogs for Streptolysin, but you can read our latest blog posts.

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Contact Information

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Calculators

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

Anonymous
04/02/2016
Application: IF
Species: Other

Anonymous
04/02/2016
Application: WB
Species: Other

Anonymous
04/01/2016
Application: WB
Species: Other