Streptavidin Native Protein

Product Discontinued

Streptavidin Native Protein Summary

• Description: A full-length protein to Streptavidin.

  Source: Streptomyces avidinii

• Protein/Peptide Type: Native Protein
• Purity: >90% pure
• Endotoxin Note: Not Tested

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Streptvidin-PE has been tested by flow cytometric analysis to detect biotinylated primary antibodies. This can be used at less than or equal to 0.06 ug per test; a test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically, but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performatnce in the assay fo interest. The streptavidin fluorochrome conjugates are commonly used in the indirect staining protocols to detect biotinylated primary antibodies in flowcytometry.
• Theoretical MW: 292.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: >90% pure

Alternate Names for Streptavidin Native Protein

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.