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Streptavidin-Phycoerythrin Summary

Used as a secondary reagent in immunofluorescent assays using biotinylated primary labeling reagents. This product has been optimized for use with monoclonal antibody-biotin reagents.


  • Flow Cytometry

Packaging, Storage & Formulations

Store at 4C. Do not freeze.


Streptavidin, a protein of 55,000 Daltons, is derived from Streptomyces avidinii and can bind 4 moles of biotin per mole of protein. The dissociation constant for biotin is approximately 10-15 M. The streptavidin-biotin complex is stable over a wide range of pH and temperatures. Streptavidin lacks carbohydrate residues present in the avidin molecule. This tends to reduce non-specific interactions with surface molecules and, therefore, streptavidin is preferred over avidin in many immunologic assays. Streptavidin can be covalently conjugated to fluorescent dyes and then used as a developer where the primary reagent was biotinylated.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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