Streptavidin Antibody [HRP]

Western Blot: Streptavidin Antibody [HRP] [NB120-7239] - Lane 1: trypsin inhibitor reduced. Lane 2: trypsin inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: 1ug per lane. Primary antibody: primary antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 1.5 hours at RT. Block: overnight at 4C. Other band(s): none.

Product Discontinued

Streptavidin Antibody [HRP] Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Streptavidin [Streptomyces avidinii]
• Localization: Cytoplasmic
• Specificity: No cross reactivity occurs against avidin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Western Blot 1:100-1:2000
• Application Notes: This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassays should be determined by researcher.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Multi-step

Notes

Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Streptavidin Antibody [HRP]

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Streptavidin Antibody (NB120-7239)(1)

Publication using NB120-7239

• Urich E, Schmucki R, Ruderisch N et al. Cargo Delivery into the Brain by in vivo identified Transport Peptides. Sci Rep 2015-01-01 [PMID: 26411801] (ELISA)