Streptavidin Antibody [HRP]

Images

 
Western Blot: Streptavidin Antibody [HRP] [NB120-7239] - Lane 1: trypsin inhibitor reduced. Lane 2: trypsin inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: 1ug per lane. ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
10.0 mg/ml

Order Details

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Streptavidin Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Streptavidin [Streptomyces avidinii]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:20000-1:100000
Readout System
Publications
Read Publication using NB120-7239.

Reactivity Notes

All Species. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against Avidin.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
10.0 mg/ml
Purity
Delipidation and Defibrination

Alternate Names for Streptavidin Antibody [HRP]

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2
  • Streptavidin

Background

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Streptavidin Antibody (NB120-7239)(1)

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(1)
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Filter By Species
All Species

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Video Protocols

WB Video Protocol

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