Streptavidin Antibody [HRP]


Western Blot: Streptavidin Antibody [HRP] [NB120-7239] - Lane 1: trypsin inhibitor reduced. Lane 2: trypsin inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: 1ug per lane. more

Product Details

Reactivity AllSpecies Glossary
Applications WB, ELISA
10.0 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

Streptavidin Antibody [HRP] Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Streptavidin [Streptomyces avidinii]
Delipidation and Defibrination
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Gentamicin Sulfate
10.0 mg/ml
Delipidation and Defibrination


  • Western Blot 1:100-1:2000
  • ELISA 1:20000-1:100000
Application Notes
This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:20,000 - 1:100,000 is suggested for this product.
Readout System
Read Publication using NB120-7239.

Reactivity Notes

All Species. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against Avidin.

Alternate Names for Streptavidin Antibody [HRP]

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2


Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Streptavidin Antibody (NB120-7239)(1)

We have publications tested in 1 application: ELISA.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Streptavidin Antibody (NB120-7239) (0)

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Secondary Antibodies


Isotype Controls

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