Streptavidin Antibody [FITC]

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Western Blot: Streptavidin Antibody [FITC] [NB120-7238] - To detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Order Details

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Streptavidin Antibody [FITC] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Streptavidin [Streptomyces avidinii]
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1:2000-1:10000

Reactivity Notes

All Species. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against Avidin.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Potassium Phosphate (pH 7.2), 150 mM NaCl, and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Streptavidin Antibody [FITC]

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2
  • Streptavidin

Background

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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