Streptavidin Antibody (S10D4)

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
S10D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Streptavidin Antibody (S10D4) Summary

Immunogen
Streptavidin.
Specificity
Does not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction. Bind to streptavidin without interfering with the biotin-binding reaction.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
WB: Use at an assay dependent dilution. Predicted molecular weight: 19 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

All Species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Streptavidin Antibody (S10D4)

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2
  • Streptavidin

Background

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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