Streptavidin Antibody (1C2) - BSA Free

Western Blot: Streptavidin Antibody (1C2) [NBP1-04345] - Recombinant Streptavidin protein (17kDa) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-04345 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Streptavidin protein

Product Discontinued

Streptavidin Antibody (1C2) - BSA Free Summary

• Immunogen: Recombinant Streptavidin (25-183aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000-1:3000

Reactivity Notes

Streptomyces avidinii

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Streptavidin Antibody (1C2) - BSA Free

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.