Streptavidin-Allophycocyanin

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Summary

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Streptavidin-Allophycocyanin Summary

Description
Used as a secondary reagent in immunofluorescent assays using biotinylated primary labeling reagents. This product has been optimized for use with biotin-conjugated monoclonal antibodies.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Background

Streptavidin, a protein of 55,000 Daltons, is derived from Streptomyces avidinii and can bind 4 moles of biotin per mole of protein. The dissociation constant for biotin is approximately 10-15 M. The streptavidin-biotin complex is stable over a wide range of pH and temperatures. Streptavidin lacks carbohydrate residues present in the avidin molecule. This tends to reduce non-specific interactions with surface molecules and, therefore, streptavidin is preferred over avidin in many immunologic assays. Streptavidin can be covalently conjugated to fluorescent dyes and then used as a developer where the primary reagent was biotinylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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