| Description | Streptavidin is purified from the bacterium Streptomyces avidinii. It is a tetrameric protein that is composed of identical subunits, with the tetramer having a molecular weight of approximately 53kDa. Each subunit binds one biotin molecule with a very high degree of specificity and affinity. The Kd for this interaction is in the region of 10-15M. Alkaline Phosphatase is a hydrolase enzyme. It is extracted from bovine intestine and purified using multiple precipitation, fractionation, chromatography, and filtration steps. Alkaline Phosphatase is frequently employed in immunoassays, and a commonly used colorimetric substrate for it is PNPP; the product of this reaction, p-nitrophenol, can be read at 405nm. Fluorogenic substrates such as 4- methylumbelliferyl phosphate (MUP) may also be used. Our streptavidin conjugates are produced using our Lightning-Link bio-conjugation technology, therefore providing high quality, stable covalent conjugates for use in your application. |
| Source | E. coli |
| Protein/Peptide Type | Protein |
| Storage | Store at -20 °C. |
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