Stomach Matched Tumor and Normal Tissue Lysate Summary
1 x Human stomach tumor tissue lysate (1 mg/ml, 100 ug/vial)
1 x Human stomach normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate SetDiagnosis: Adenocarcinoma, poorly differentiated
Stage: II, T3N0M0
Tumor Pathology Data
Gross findings: Ulcerative lesion located at the antrum, ill demarcated. Ulceration measures 4 cm in diameter. Cut section is firm and white.
Tissue sections show proliferation of malignant cells. The epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm, Cytoplasm contains much mucin. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are not evident. The neoplastic cells form groups and lines invading the smooth muscle propria and serosa. The stroma is infiltrated by small numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Blood vessel invasions are not revealed. Lymph nodes show hyperplasia.
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Array
Matched Tumor and Normal Tissue
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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