Staphylococcus Enterotoxin Antibody (S2)

Product Discontinued

Staphylococcus Enterotoxin Antibody (S2) Summary

• Immunogen: Full length native protein (purified from FRI-722H strain of S. aureus).
• Localization: Secreted
• Specificity: Enterotoxins A and D of Staphylococcus aureus. Specificity was established using standard staphylococcal entertoxins A, B, C1, C2, D, E coated on ELISA plates and appropriate dilution of ascitic fluids.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000

Reactivity Notes

Cross-reacts with S. aureus.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Staphylococcus Enterotoxin Antibody (S2)

• Ent A
• EntA
• Enterotoxin A
• Enterotoxin type A
• SEA
• Staphylococcus enterotoxin type A

Background

Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.