Staphylococcus Aureus Enterotoxin Type A Antibody

Western Blot: Staphylococcus Aureus Enterotoxin Type A Antibody [NBP2-75673] - Analysis of Enterotoxin A on recombinant protein lysate using anti-Enterotoxin A antibody at 1/1,000 dilution.

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Staphylococcus Aureus Enterotoxin Type A Antibody Summary

• Immunogen: Recombinant protein within Staphylococcus Aureus Enterotoxin Type A aa 1-257 / 257. (SwissProt: P0A0L2 StaphylococcusAureus)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Western Blot 1:500-1:1000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Staphylococcus Aureus Enterotoxin Type A Antibody

• Ent A
• EntA
• Enterotoxin A
• Enterotoxin type A
• SEA
• Staphylococcus enterotoxin type A

Background

Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.