Staphylococcus Aureus Enterotoxin Type A Antibody (C4)

Product Discontinued

Staphylococcus Aureus Enterotoxin Type A Antibody (C4) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with S. aureus enterotoxin type A.
• Specificity: Staphylococcus aureus Enterotoxin type A (C4)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Recommended pairs for sandwich immunoassay (capture-detection): NB100-73023, NB100-73024, NB100-73019, NB100-73022, or NB100-73020 as capture with NB110-59900 for detection; or use with NB110-59900 as capture and NB100-73023 or NB100-73019 for detection.

Reactivity Notes

This antibody is reactive against S. aureus Enterotoxin type A.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Staphylococcus Aureus Enterotoxin Type A Antibody (C4)

• Ent A
• EntA
• Enterotoxin A
• Enterotoxin type A
• SEA
• Staphylococcus enterotoxin type A

Background

Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Staphylococcus Aureus Enterotoxin Type A Antibody (NB110-59900)(1)

Publication using NB110-59900

• Ochsner UA, Bell SJ, O'Leary AL et al. Inhibitory effect of REP3123 on toxin spore formation in Clostridium difficile, in vivo efficacy in a hamster gastrointestinal infection model. J Antimicrob Chemother 2009;63(5):964-971. [PMID: 19251726]