SSEA-3 Antibody (MC-631) [PE]

Images

 
There are currently no images for SSEA-3 Antibody (NB100-1832PE).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
MC-631
Clonality
Monoclonal
Host
Rat

SSEA-3 Antibody (MC-631) [PE] Summary

Immunogen
4-8 cell stage mouse embryos.
Marker
Embryonic Stem Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Rat
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
PE
Format
PE (A=488, E=575)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
IgM purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 10-20 ug/ml
Application Notes
This SSEA3 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin sections, Immunoprecipitation and Western Blot.

Reactivity Notes

This antibody recognizes the SSEA3 that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.

Alternate Names for SSEA-3 Antibody (MC-631) [PE]

  • SSEA3
  • SSEA-3
  • Stage specific embryonic antigen 3
  • Stage-specific embryonic antigen 3

Background

SSEA-3 (stage-specific embryonic antigen-3) is a member of SSEAs which are present on eggs as well as preimplantation embryos at specific stages of early development. SSEAs are glycan epitopes which resides on glycoproteins/glycolipids of mammalian cells, and the epitope of several of these antigens consists solely of sugar residues. SSEA-3 antigen was identified following immunization of rats with 4-8 cell mouse embryos and was present on glycolipids of human teratocarcinoma cells. During protein glycosylation, beta-1,3-galactosyltransferase 5 catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing glycolipid GB5 which is SSEA-3. In its functions. SSEA-3 has been proposed to involve in the process of cell differentiation. SSEA3 expression is mostly restricted to the cytoplasm or apical surface of epithelial cells and it has been reported to express in various normal tissues with high expression levels in kidney. SSEA3 has been reported to be expressed in the ascites fluids from patients of hepatoma or pancreatic cancer, breast cancer including breast cancer stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-3 Antibody (NB100-1832PE) (0)

There are no publications for SSEA-3 Antibody (NB100-1832PE).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SSEA-3 Antibody (NB100-1832PE) (0)

There are no reviews for SSEA-3 Antibody (NB100-1832PE). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SSEA-3 Antibody (NB100-1832PE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-1832APC
Biotin Labeled NB100-1832B
DyLight 350 Labeled NB100-1832UV
DyLight 405 Labeled NB100-1832V
DyLight 405LS Labeled NB100-1832V3
DyLight 488 Labeled NB100-1832G
DyLight 550 Labeled NB100-1832R
DyLight 650 Labeled NB100-1832C
DyLight 680 Labeled NB100-1832FR
DyLight 755 Labeled NB100-1832IR
FITC Labeled NB100-1832F
HRP Labeled NB100-1832H
PerCP Labeled NB100-1832PCP

Additional SSEA-3 Antibody (MC-631) [PE] Products

SSEA-3 NB100-1832PE

Research Areas for SSEA-3 Antibody (NB100-1832PE)

Find related products by research area.

Blogs on SSEA-3

There are no specific blogs for SSEA-3, but you can read our latest blog posts.

Contact Information

Product PDFs

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-1832PE below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought