SSEA-3 Antibody (MC-631) [Janelia Fluor® 646]

Product Discontinued

SSEA-3 Antibody (MC-631) [Janelia Fluor® 646] Summary

• Immunogen: 4-8 cell stage mouse embryos.
• Marker: Embryonic Stem Cell Marker
• Specificity: Recognizes a carbohydrate epitope of SSEA-3
• Isotype: IgM
• Clonality: Monoclonal
• Host: Rat
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

This antibody recognizes the SSEA3 that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SSEA-3 Antibody (MC-631) [Janelia Fluor® 646]

• SSEA3
• SSEA-3
• Stage specific embryonic antigen 3
• Stage-specific embryonic antigen 3

Background

SSEA3 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.