SPRYD4 Antibody - BSA Free

Western Blot: SPRYD4 Antibody [NBP1-77100] - Analysis in fetal human liver tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide

Immunocytochemistry/Immunofluorescence: SPRYD4 Antibody [NBP1-77100] - Immunofluorescence of SPRYD4 in human kidney tissue with SPRYD4 antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: SPRYD4 Antibody [NBP1-77100] - Immunohistochemistry of SPRYD4 in human kidney tissue with SPRYD4 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SPRYD4 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the center of human SPRYD4. The immunogen is located within amino acids 70 - 120 of SPRYD4. Amino Acid Squence: VTSGRHYWEVTVKRSQQF
• Specificity: SPRYD4 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD4 are known to exist; this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPRYD4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  SPRYD4 Peptide (NBP1-77100PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%), Porcine (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SPRYD4 Antibody - BSA Free

• DKFZp686N0877
• SPRY domain containing 4
• SPRY domain-containing protein 4

Background

The SPRY domain-containing protein 4 (SPRYD4) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD4 is ubiquitously expressed and is most abundant in kidney, brain, bladder, thymus and stomach. Little is known of the function of the SPRYD4 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPRYD4
• Uniprot:
  • NP_997227(Human)