| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Concentration | 2.0 mg/ml |
| Description | We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity | Membrane Protein - Human Adult Normal Tissue: Spinal Cord |
| Dilutions |
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| Application Notes | Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration | 2.0 mg/ml |
| Type | Tissue |
| Life Stage | Adult |
| Tissue | Nervous |
| Protein State | Native |
| Tissue Condition | Normal |
| Subcellular Fraction | Membrane |
|
Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group ... Read full blog post. |
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