Recombinant SARS-CoV-2 Spike (RBD+SD1+SD2) His (C-Term) Protein

Western Blot: Recombinant SARS-CoV-2 Spike (RBD+SD1+SD2) His (C-Term) Protein [NBP3-07951] - Western Blot Validation with Spike Recombinant Protein. Loading: 30 ng per lane of Spike recombinant protein, NBP3-07951. Antibodies: SARS-CoV-2 (COVID-19) Spike RBD, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.

ELISA: Recombinant SARS-CoV-2 Spike (RBD+SD1+SD2) His (C-Term) Protein [NBP3-07951] - Binding Assay of ACE2 and Spike Recombinant Protein. Coating: Spike recombinant protein (NBP3-07951) from 0.3 ng to 1000 ng. Capture: ACE2 recombinant protein (0.5 ug/mL). Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.

ELISA: Recombinant SARS-CoV-2 Spike (RBD+SD1+SD2) His (C-Term) Protein [NBP3-07951] - ELISA Validation of Spike Recombinant Protein. Coating Antigen: Spike recombinant protein (NBP3-07951) from 0.3 ng to 1000 ng. Capture Antibody: SARS-CoV-2 (COVID-19) Spike RBD antibody (1 ug/mL). Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.

SDS-Page: Recombinant SARS-CoV-2 Spike (RBD+SD1+SD2) His (C-Term) Protein [NBP3-07951] - SDS-PAGE Validation with Spike Recombinant Protein. Loading: 1 ug Spike recombinant protein, NBP3-07951. Observed: 70kD

• Reactivity: V
• Applications: WB, ELISA, PAGE
• Concentration: 1 mg/ml

Recombinant SARS-CoV-2 Spike (RBD+SD1+SD2) His (C-Term) Protein Summary

• Description: A recombinant protein with a C-terminal His-tag corresponding to amino acids Glu309-Ala684 SARS-CoV-2 Spike Protein
  
  

  Source: HEK293

• Source: HEK293
• Protein/Peptide Type: Recombinant Protein
• Purity: >90%

Applications/Dilutions

• Dilutions:
  • ELISA
  • SDS-Page
  • Western Blot
• Theoretical MW: 42.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -70C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.