Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric) Summary
This assay has high sensitivity and excellent specificity for detection of human sTWEAK. No significant cross-reactivity or interference between human sTWEAK and analogues was observed.
|Standard Curve Range
0.312 ng/ml-20 ng/ml
%CV < 10
%CV < 8
50 - 100 uL
ELISA Kit (Colorimetric)
Packaging, Storage & Formulations
- Adhesive Strip (For 96 wells)
- Assay plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
Please contact our Technical Services with any questions regarding species reactivity
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTWEAK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTWEAK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTWEAK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTWEAK bound in the initial step. The color development is stopped and the intensity of the color is measured.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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