Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric)

ELISA: Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP2-60145] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: serum, plasma, tissue homogenates
• Standard Curve Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml

Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of human sTWEAK. No significant cross-reactivity or interference between human sTWEAK and analogues was observed.
• Standard Curve Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: 0.078 ng/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTWEAK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTWEAK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTWEAK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTWEAK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.