Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Images

 
ELISA: Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP2-60145] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma, tissue homogenates
Standard Curve Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml

Order Details

Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human sTWEAK. No significant cross-reactivity or interference between human sTWEAK and analogues was observed.
Standard Curve Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTWEAK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTWEAK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTWEAK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTWEAK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Soluble TWEAK/TNFSF12 ELISA Kit (NBP2-60145) (0)

There are no publications for Soluble TWEAK/TNFSF12 ELISA Kit (NBP2-60145).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Soluble TWEAK/TNFSF12 ELISA Kit (NBP2-60145) (0)

There are no reviews for Soluble TWEAK/TNFSF12 ELISA Kit (NBP2-60145). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Soluble TWEAK/TNFSF12 ELISA Kit (NBP2-60145) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Soluble TWEAK/TNFSF12 Products

Blogs on Soluble TWEAK/TNFSF12

There are no specific blogs for Soluble TWEAK/TNFSF12, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Human Soluble TWEAK/TNFSF12 ELISA Kit (Colorimetric) and receive a gift card or discount.