Smith Antigen ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
12.5 to 200 U/mL
Sensitivity
1.0 U/mL

Order Details

Smith Antigen ELISA Kit (Colorimetric) Summary

Standard Curve Range
12.5 to 200 U/mL
Sensitivity
1.0 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 1.3-5.3%
Intra-Assay
CV% < 2.0-4.1%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
Sm Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against Sm in human serum or plasma.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow,concentrate (5x).
  2. Stop solution, contains acid. Ready to use.
  3. TMB substrate, containing 3,3’,5,5’- Tetramethylbenzidine, colorless. Ready to use.
  4. Wash solution, containing Tris, detergent, preservative NaN3 0.09%, 50x concentrate.
  5. Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
  6. Enzyme conjugate, containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative Proclin 300 0.05%, light red. Ready to use.
  7. Calibrators A-F (0, 12.5, 25, 50, 100, 200 U/mL), containing Sm antibodies in aserum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use.
  8. Control positive (1) and negative (2), containing Sm antibodies in a serum/buffer matrix(PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. The concentration isspecified on the certificate of analysis.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Smith Antigen ELISA Kit (Colorimetric)

  • Smith antigen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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