Smith Antigen ELISA Kit (Colorimetric) Summary
| Description |
Sm Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against Sm in human serum or plasma. |
| Standard Curve Range |
12.5 to 200 U/mL |
| Sensitivity |
1.0 U/mL |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 1.3-5.3% |
| Intra-Assay |
CV% < 2.0-4.1% |
| Sample Volume |
100 uL (prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- Calibrators A-F (0, 12.5, 25, 50, 100, 200 U/mL), containing Sm antibodies in aserum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use.
- Control positive (1) and negative (2), containing Sm antibodies in a serum/buffer matrix(PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. The concentration isspecified on the certificate of analysis.
- Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
- Enzyme conjugate, containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative Proclin 300 0.05%, light red. Ready to use.
- Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow,concentrate (5x).
- Stop solution, contains acid. Ready to use.
- TMB substrate, containing 3,3’,5,5’- Tetramethylbenzidine, colorless. Ready to use.
- Wash solution, containing Tris, detergent, preservative NaN3 0.09%, 50x concentrate.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Smith Antigen ELISA Kit (Colorimetric)
Background
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Smith Antigen ELISA Kit (KA1112). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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