Sialic Acid Assay Kit (Colorimetric/Fluorometric)

Sialic Acid Assay Kit (Fluorometric) [KA1655] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

• Reactivity: Bv
• Applications: Func, NULL
• Suitable Sample Type: Biological Samples (e.g. Milk, Plasma, Saliva, Serum)
• Standard Curve Range: Colorimetric Assay: 5 to 1000 uM, Fluorometric Assay: 0.5 to 100 uM
• Sensitivity: OD, FL: 5, 0.5 uM

Sialic Acid Assay Kit (Colorimetric/Fluorometric) Summary

• Description: Quality control test: Standard Curve Sialic Acid Assay Kit is a quantitative determination of free and total sialic acid.
• Specificity: This kit can be used with both colorimetric and fluorometric detection systems.
• Standard Curve Range: Colorimetric Assay: 5 to 1000 uM, Fluorometric Assay: 0.5 to 100 uM
• Sensitivity: OD, FL: 5, 0.5 uM
• Assay Type: Colorimetric/Fluorometric
• Sample Volume: 60 uL
• Kit Type: Assay Kit (Colorimetric/Fluorometric)

Applications/Dilutions

• Dilutions:
  • Functional
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Sialic Acid is a general name for nine carbon acidic sugars with N- or O- substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Sialic Acid Kit (KA1655)(3)

Publications using KA1655

• Xu W, Mingyue L, Jifeng Z et al. CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder. Cell Metab. 2022-08-02 [PMID: 35921817]
• Sharma R, Ahlawat S, Sharma H et al. Variable sialic acid content in milk of Indian cattle and buffalo across different stages of lactation. J Dairy Res 2018-12-06 [PMID: 30520408]
• Novel pH-dependent regulation of Human cytosolic sialidase 2 (NEU2) activities by siastatin b and structural prediction of neu2/siastatin b complex. MM Rahmana, T Hirokawab, D Tsujia et al. Biochem Biophys Rep [PMID: 29124209]