Sheep IgG, Fab2 Native Protein

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Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, ELISA, IHC
Concentration
2.0 mg/ml

Order Details

Sheep IgG, Fab2 Native Protein Summary

Description
Native Sheep IgG, Fab2 protein.

Source: Sheep Serum

Protein/Peptide Type
Native Protein
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Background

Sheep IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. Sheep IgG F(ab')2 molecules lack the Fc portion of Sheep IgG and therefore receptors that bind Sheep IgG Fc will not bind Sheep IgG F(ab')2 molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Sheep IgG, Fab2 NBP1-97013

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