Rabbit anti-Monkey Serum Proteins Secondary Antibody

• Reactivity: Pm
• Applications: ELISA, IP, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Rabbit anti-Monkey Serum Proteins Secondary Antibody Summary

• Description: The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.
• Immunogen: Pooled whole Rhesus monkey (Macaca mulatta) serum and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Precipitating polyclonal Rabbit antiserum to Monkey Serum Proteins.
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoelectrophoresis
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: Precipitation assays. In immunoelectrophoresis use 2 ul serum or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in 3 mm diameter center well and 2 ul serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
  
  Precipitation assays.

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross reactivity. Consequently it is not species-specific. It will also react with other old-world Monkeys, including Cercopithecus, Cynomolgus and baboon. This antiserum is also reacting with Chimpanzee, but it gives less precipitin lines in an immunoelectrophoresis than with Rhesus Monkey Serum.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Whole Antiserum
• Reconstitution Instructions: Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.