Serotonin ELISA Kit (Colorimetric)

ELISA: Serotonin ELISA Kit (Colorimetric) [NBP2-68127] - Samples were spiked with high concentrations of Serotonin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Serotonin ELISA Kit (Colorimetric) [NBP2-68127] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 ng/mL
• Sensitivity: 9.38 ng/mL

Serotonin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes ST/5-HT in samples. No significant cross-reactivity or interference between ST/5-HT and analogues was observed.
• Standard Curve Range: 15.63 - 1000 ng/mL
• Sensitivity: 9.38 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 5.06%
• Intra-Assay: CV% < 5.11%
• Spike Recovery: 86-107%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Serotonin ELISA Kit (Colorimetric)

• 5-HT
• 5-hydroxytryptamne
• Human 5-HT elisa kit

Background

Serotonin-positive cells are present in gastric, duodenal, jejunal, ileal, colonic, and appendiceal mucosae. Positive cells are sparse and usually occur in the lower half of the gastric and intestinal mucosae. In the appendix, in addition to the mucosal serotonin-containing cells, there are single or small clusters of serotonin-positive cells in the sub-epithelial region of the lamina propria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.