Human Seminal Vesicle Whole Tissue Lysate (Adult Whole Normal)

• Reactivity: Hu
• Applications: WB
• Concentration: 5.0 mg/ml

Human Seminal Vesicle Whole Tissue Lysate (Adult Whole Normal) Summary

• Description: We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
• Specificity: Total Protein - Human Adult Normal Tissue: Seminal Vesicle

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Reproductive
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Seminal Vesicle Lysate (NB820-59252). (Showing 1 - 1 of 1 FAQ).

1. I recently ordered whole normal skeletal muscle lysate and whole normal liver lysate (NB820-59253 and 59232). What are the exact concentrations for the buffer components that each of the lysates is in? I need to dilute the lysate because the concentration given is too high (5mg/mL), but I want to dilute it in whatever buffer it is in. Can you recommend what I should do?
   • Unfortunately the concentrations of the components (stated on the datasheet) are proprietary. I apologize. If the concentration is too high, our lab recommends simply diluting it in your loading buffer. A 4x or higher loading buffer should suffice.