Human Semaglutide Antibody ELISA Kit (Colorimetric)

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ELISA: Human Semaglutide Antibody ELISA Kit (Colorimetric) [NBP3-45153] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell culture supernatant, cell or tissue lysate
Standard Curve Range
0.781 - 50 ng/mL (example only; lot dependent)
Sensitivity
0.469 ng/mL (example only; lot dependent)

Order Details

Human Semaglutide Antibody ELISA Kit (Colorimetric) Summary

Description
Assay Length: 3 hours
Specificity
Specifically binds with Anti-Semaglutide, no obvious cross reaction with other analogues.
Standard Curve Range
0.781 - 50 ng/mL (example only; lot dependent)
Sensitivity
0.469 ng/mL (example only; lot dependent)
Assay Type
Indirect ELISA
Inter-Assay
%CV < 5.53 (example only; lot dependent)
Intra-Assay
%CV < 5.9 (example only; lot dependent)
Spike Recovery
85 - 103% (example only; lot dependent)
Sample Volume
3 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Antibody Dilution Buffer
  2. Biotin-labeled Antibody (Concentrated 100x)
  3. ELISA Microplate (Dismountable)
  4. HRP-Streptavidin Conjugate (SABC 100x)
  5. Lyophilized Standard
  6. Plate Sealer
  7. Product Description
  8. SABC Dilution Buffer
  9. Sample Dilution Buffer
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25x)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Semaglutide Antibody ELISA Kit (NBP3-45153). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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