SARS-CoV NSP8 Antibody


Western Blot: SARS-CoV NSP8 Antibody [NBP2-89180] - mmunoprecipitation followed by western blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). more
Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody [NBP2-89180] - Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG was used.

Product Details

Reactivity V, VSpecies Glossary
Applications WB, ICC/IF, IM, IP
85 mg/ml

Order Details

SARS-CoV NSP8 Antibody Summary

This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Predicted Species
SARS-CoV-2 (97%). Backed by our 100% Guarantee.
Sterile filtered
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  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:300
  • Immunomicroscopy 1:100
  • Immunoprecipitation 1:60
Application Notes
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degrees C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Reactivity Notes

Expected species cross reactivity based on immunogen sequence (full length) homology: COVID-19 (97%)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.01% Sodium Azide
85 mg/ml
Sterile filtered


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Additional SARS-CoV NSP8 Products

Array NBP2-89180

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