SARS-CoV NSP8 Antibody

Images

 
Western Blot: SARS-CoV NSP8 Antibody [NBP2-89180] - mmunoprecipitation followed by western blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). ...read more
Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody [NBP2-89180] - Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG was used.

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ICC/IF, IM, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
85 mg/ml

Order Details

SARS-CoV NSP8 Antibody Summary

Immunogen
This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Predicted Species
SARS-CoV-2 (97%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Purity
Sterile filtered
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:300
  • Immunomicroscopy 1:100
  • Immunoprecipitation 1:60
Application Notes
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degrees C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Reactivity Notes

Expected species cross reactivity based on immunogen sequence (full length) homology: COVID-19 (97%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative
0.01% Sodium Azide
Concentration
85 mg/ml
Purity
Sterile filtered

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS-CoV NSP8 Antibody (NBP2-89180) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SARS-CoV NSP8 Products

Array NBP2-89180

Blogs on SARS-CoV NSP8

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Contact Information

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