SARS-CoV NSP8 Antibody


Western Blot: SARS-CoV NSP8 Antibody [NBP2-89180] - mmunoprecipitation followed by western blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). more
Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody [NBP2-89180] - Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG was used.

Product Details

Reactivity V, VSpecies Glossary
Applications WB, ICC/IF, IM, IP

Order Details

SARS-CoV NSP8 Antibody Summary

This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Predicted Species
SARS-CoV-2 (97%). Backed by our 100% Guarantee.
Sterile filtered
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:300
  • Immunomicroscopy 1:100
  • Immunoprecipitation 1:60
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mM HEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
Theoretical MW
21.87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP2-89180.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (97%)

Packaging, Storage & Formulations

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.01% Sodium Azide
Sterile filtered


Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for SARS-CoV NSP8 Antibody

  • COVID-19
  • NSP8


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS-CoV NSP8 Antibody (NBP2-89180)(1)

We have publications tested in 1 application: WB.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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