SARS-CoV NSP8 Antibody

Images

 
Western Blot: SARS-CoV NSP8 Antibody [NBP2-89180] - mmunoprecipitation followed by western blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). ...read more
Immunocytochemistry/ Immunofluorescence: SARS-CoV NSP8 Antibody [NBP2-89180] - Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG was used.

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ICC/IF, IM, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SARS-CoV NSP8 Antibody Summary

Immunogen
This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Predicted Species
SARS-CoV-2 (97%). Backed by our 100% Guarantee.
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Purity
Sterile filtered
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:300
  • Immunomicroscopy 1:100
  • Immunoprecipitation 1:60
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mM HEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
Theoretical MW
21.87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-89180.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (97%)

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
0.01% Sodium Azide
Purity
Sterile filtered

Notes

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for SARS-CoV NSP8 Antibody

  • COVID-19
  • NSP8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS-CoV NSP8 Antibody (NBP2-89180)(1)

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
All Species

Reviews for SARS-CoV NSP8 Antibody (NBP2-89180) (0)

There are no reviews for SARS-CoV NSP8 Antibody (NBP2-89180). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS-CoV NSP8 Antibody (NBP2-89180) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SARS-CoV NSP8 Antibody and receive a gift card or discount.