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SARS-CoV nsp13 Antibody

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Immunocytochemistry/ Immunofluorescence: SARS-CoV nsp13 Antibody [NBP2-89168] - Immunofluorescence microscopy using anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated ...read more

Product Details

Summary
Product Discontinued
View other related SARS-CoV nsp13 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-89168
    • Availability
      Product Discontinued

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SARS-CoV nsp13 Antibody Summary

Immunogen
The antibody was developed by immunizing rabbits with a BSA-coupled synthetic peptide corresponding to amino acids 584-604 (C-klqftsleiprrnvatlq) from the N (Nonstructural protein 13) for the Human SARS coronavirus.
Specificity
This antibody is directed against SARS-Coronavirus nsp13 protein.
Predicted Species
SARS-CoV-2 (100%). Backed by our 100% Guarantee.
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
orf1ab
Purity
Whole Antiserum
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:800
  • Immunoprecipitation 1:60
  • Western Blot 1:1000
Application Notes
This antibody has been tested for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
Theoretical MW
66.87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-89168.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (100%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
0.01% Sodium Azide
Purity
Whole Antiserum

Notes

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for SARS-CoV nsp13 Antibody

  • pp1a
  • Replicase polyprotein 1a
  • Sars-Cov Nonstructural Protein 13

Background

The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS-CoV nsp13 Antibody (NBP2-89168)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol orf1ab