SARS-CoV nsp13 Antibody

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Immunocytochemistry/ Immunofluorescence: SARS-CoV nsp13 Antibody [NBP2-89168] - Immunofluorescence microscopy using Rockland's anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated ...read more

Product Details

Summary
Reactivity V, VSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
85 mg/ml

Order Details

SARS-CoV nsp13 Antibody Summary

Immunogen
The antibody was developed by immunizing rabbits with a BSA-coupled synthetic peptide corresponding to amino acids 584-604 (C-klqftsleiprrnvatlq) from the N (Nonstructural protein 13) for the Human SARS coronavirus.
Specificity
This antibody is directed against SARS-Coronavirus nsp13 protein.
Predicted Species
SARS-CoV-2 (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
orf1ab
Purity
Whole Antiserum
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:800
  • Immunoprecipitation 1:60
Application Notes
This antibody has been tested for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degrees C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Reactivity Notes

Expected species cross reactivity based on immunogen sequence homology: COVID-19 (100%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole Antiserum
Preservative
0.01% Sodium Azide
Concentration
85 mg/ml
Purity
Whole Antiserum

Alternate Names for SARS-CoV nsp13 Antibody

  • pp1a
  • Replicase polyprotein 1a
  • Sars-Cov Nonstructural Protein 13

Background

The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS-CoV nsp13 Antibody (NBP2-89168) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SARS-CoV nsp13 Products

Array NBP2-89168

Bioinformatics Tool for SARS-CoV nsp13 Antibody (NBP2-89168)

Discover related pathways, diseases and genes to SARS-CoV nsp13 Antibody (NBP2-89168). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on SARS-CoV nsp13

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol orf1ab