Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein

SDS-Page: Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein [NBP3-07083] - SDS-PAGE Image

• Applications: PAGE

Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein Summary

• Description: A recombinant protein with a His (N-Term) tag corresponding to the amino acids Ala1-Gly638 of SARS-CoV-2 nsp2
  
  Source: E.coli
  
  Accession: YP_009725298.1
• Protein/Peptide Type: Recombinant Protein
• Gene: ORF1ab
• Purity: >90%, by SDS-PAGE quantitative densitometry by Coomassie Blue Staining

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 72.81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.5
• Preservative: No Preservative
• Purity: >90%, by SDS-PAGE quantitative densitometry by Coomassie Blue Staining

Alternate Names for Recombinant SARS-CoV-2 nsp2 His (N-Term) Protein

• Non-structural protein 2
• NSP2
• p65 homolog

Background

SARS-CoV-2 Nonstructural Protein 2 (NSP2) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP2 is 638 amino acids (aa) with a theoretical molecular weight of 70.5 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP2 has 68.3% sequence identity and 90% sequence similarity (1). SARS-CoV-2 NSP2 is shown to bind two host proteins, prohibitin 1 (PHB1) and prohibitin 2 (PHB2) (1). The NSP2-PHB protein interaction indicates that SARS-CoV-2 NBP2 might function in disturbing the host-cell environment during viral infection (1). A SARS-CoV-2 interactome study revealed that NSP2 has a role in vesicle trafficking, specifically reconfiguring endoplasmic reticulum and Golgi trafficking during viral infection via the WASH complex (Wiskott Aldrich Syndrome protein and scar homologue complex) (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ORF1ab