Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) Protein

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SDS-Page: Recombinant Virus SARS-CoV-2 3CL Protease Avi Epitope Tag His (N-Term) Protein [NBP2-92992] - Recombinant Virus SARS-CoV-2 3CL Protease Avi Epitope Tag Protein [NBP2-92992] - Recombinant Virus SARS-CoV-2 3CL ...read more

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Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) Protein Summary

Description
Recombinant Virus SARS-CoV-2 3CL Protease is produced by E. coli expression system. The target protein is expressed with sequence (Ser1-Gln306) of 2019-ncov 3C-like Proteinase (Accession #YP_009725301.1) fused with a 6×His,Avi tag at the N-terminus.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1.0 EU/ug of the protein by LAL method.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
Supplied as a 0.22 um filtered solution in 20mM HEPES, 120mM NaCl, 10% Glycerol, 2mM TCEP, pH7.4
Preservative
No Preservative
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) Protein

  • 3CL Protease
  • 3CL Proteinase
  • 3C-like main protease
  • 3CL-Mpro
  • COVID-19
  • M Proteinase
  • Main Protease
  • Mpro

Background

Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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