Salmonella Enteritidis Antibody (10D9H (E91))

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA
Clone
10D9H (E91)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Salmonella Enteritidis Antibody (10D9H (E91)) Summary

Immunogen
Heat inactivated cells from Salmonella (LPS groups A, B & D).
Specificity
This antibody recognises a common antigenic determinant that is found in the LPS of Salmonella enteriditis (Group D), Salmonella typhimurium (Group B) and Salmonella paratyphi (Group A). This antibody is thought to recognise the '0-12' antigen [2-D-mannose (1-4) I- ramnose (1-3)-D galactose] of salmonella LPS. A similar sequence is found in Group E, which may account for the weak cross reactivity observed with bacteria of this group.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Background

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-66471AF350
Alexa Fluor 405 NB100-66471AF405
Alexa Fluor 488 NB100-66471AF488
Alexa Fluor 532 NB100-66471AF532
Alexa Fluor 594 NB100-66471AF594
Alexa Fluor 647 NB100-66471AF647
Alexa Fluor 700 NB100-66471AF700
Alexa Fluor 750 NB100-66471AF750
Biotin NB100-66471B
DyLight 350 NB100-66471UV
DyLight 405 NB100-66471V
DyLight 488 NB100-66471G
DyLight 550 NB100-66471R
DyLight 594 NB100-66471DL594
DyLight 650 NB100-66471C
DyLight 680 NB100-66471FR
DyLight 755 NB100-66471IR
FITC NB100-66471F
HRP NB100-66471H

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Contact Information

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Calculators

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