Salmonella Antibody (10D9H)

Product Discontinued

Salmonella Antibody (10D9H) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with heat inactivated bacterial cells of Salmonella enteriditis D, paratyphi A and typhimurium B.
• Specificity: This antibody is reactive against Sero Groups A, B and D. Binding constants (Ka, M-1) of MAbs with LPS of A, B and D groups are as follows: LPS A = 2.1 x 10^9, LPS B = 1.1 x 10^9, LPS D = 6.5 x 10^8, LPS E = 1x 10^6.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000

Reactivity Notes

This antibody detects Salmonella enteriditis D, paratyphi A and typhimurium B. It has slight reactivity with Salmonella anatum and Selandia. It does not react with other Salmonella, E. coli, or Klebsiella.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Salmonella Antibody (10D9H)

• Salmonellae

Background

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Salmonella Antibody (NB200-410) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Finny Mathew ELISA 04/25/2014

Summary

• Application: ELISA
• Lot: 12/03-SO22-9H (Order #40926)