Salmonella Antibody (10D9H)

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB
Clone
10D9H
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Salmonella Antibody (10D9H) Summary

Immunogen
Heat inactivated bacterial cells of Salmonella enteriditis D, paratyphi A and typhimurium B.
Specificity
This antibody is reacive against Sero Groups A, B and D, Antigens 1,2,3, 6 and 7. Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with heat inactivated bacterial cells of Salmonella paratyphi (group A), Salmonella typhimurium (group B) and Salmonella enteriditis (group D). Binding constants (Ka, M-1) of MAbs with LPS of A, B and D groups are as follows: LPS A = 2.1 x 10^9, LPS B = 1.1 x 10^9, LPS D = 6.5 x 10^8, LPS E = 1x 10^6.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 1 Review rated 4
using
NB200-410 in the following application:

Reactivity Notes

This antibody detects Salmonella enteriditis D, paratyphi A and typhimurium B. It has slight reactivity with Salmonella anatum and Selandia. It does not react with other Salmonella, E. coli, or Klebsiella.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Background

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Salmonella Antibody (NB200-410) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 application: ELISA.

Reviews using NB200-410:
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ELISA
(1)
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ELISA Salmonella NB200-410
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reviewed by:
Finny Mathew
ELISA 04/25/2014
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Summary

ApplicationELISA
Lot12/03-SO22-9H (Order #40926)
CommentsKPL's Anti-Salmonella CSA-1 polyclonal antibody (Cat# 01-91-99) was used as the detection antibody on the nanoparticles at 1 microgram per tube

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Recent Reviews

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Finny Mathew
04/25/2014
Application: ELISA
Species: