RuvA Antibody

Product Discontinued

RuvA Antibody Summary

• Immunogen: Purified E. coli RuvA protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Reactivity Notes

E.coli

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Serum
• Preservative: No Preservative
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Alternate Names for RuvA Antibody

• EC 3.1.22.4
• Holliday junction ATP dependent DNA helicase ruvA
• Ruv A

Background

In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.