Plant Rubisco ELISA Kit (Colorimetric)

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ELISA: Plant Rubisco ELISA Kit (Colorimetric) [NBP2-60142] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity PlSpecies Glossary
Applications ELISA
Suitable Sample Type
plant tissues
Standard Curve Range
3.12 ug/ml-800 ug/ml
Sensitivity
3.12 ug/ml

Order Details

Plant Rubisco ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of plant RuBisCO. No significant cross-reactivity or interference between plant RuBisCO and analogues was observed.
Standard Curve Range
3.12 ug/ml-800 ug/ml
Sensitivity
3.12 ug/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 20%
Intra-Assay
CV% < 10%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Sample Diluent
  2. Sample Extraction Buffer (25 x concentrate)
  3. Standard
  4. Stop Solution
  5. Wash Buffer (25 x concentrate)
  6. TMB Substrate
  7. Adhesive Strip (For 96 wells)
  8. Antibody (100 x concentrate)
  9. Antibody Diluent
  10. HRP-conjugate Diluent
  11. HRP-conjugate(100 x concentrate)
  12. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Plant Rubisco ELISA Kit (Colorimetric)

  • 5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I [Spinacia oleracea]
  • EC 2.1.1.43
  • rbcL
  • Ribulose-1

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO. The competitive inhibition reaction is launched between with pre-coated RuBisCO and RuBisCO in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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